19th-century activist Susan B. Anthony, looking a little on the stern side. The image is undated; Anthony was born in 1820, and based on her appearance here we'll hazard a guess that this photo was taken between 1860 and 1875.
The image is from the George Grantham Bain Collection in the U.S. Library of Congress, Prints and Photographs Division. (Digital ID: ggbain 30124)